Virus Precipitation
Virus Precipitation with PEG and NaCl...
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Category: Protocols
Glassware Washing Protocol for Cell Culture
How to wash glassware for cell/tissue culture Disinfect your glassware, media, and cells. Rinse...
Read MoreSFP Synthase (4´-phosphopantetheinyl transferase)
Phosphopantetheinyl Labeling of Proteins in vitro (P9302) Protocol Dissolve the vial of CoA substrate (50 nmol) in 50 μl of fresh DMSO to yield a labeling stock solution of 1 mM CoA substrate. Mix by vortexing for 10 minutes...
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Morgagni Operation
!!! Radiation Safety Rules !!! Use gloves to stain your sample and insert the holder to the...
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Common Stock Solutions
NaOH How to make a 10N & 100 ml NaOH Stock Solution Molar mass: 39.997 g/molFormula:...
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TCEP - (tris(2-carboxyethyl)phosphine
How to Make a 0.5M TCEP Stock Solution Molecular formula: C9H15O6P • HCl Molecular weight: 286.65...
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Ni-NTA Bead Regeneration
Imidazole removal - in between use/prior to storage in 20% EtOH Wash with 3-5 column volumes of 20...
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Grid Preparation for Carbon Coating
Reagents: Collodion (Parlodion Strips) 2% in Amyl acetate - EMS 12620-50 Mulberry Paper Mulberry...
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Uranyl Formate Stain Preparation
Uranyl Formate Stain Preparation for Negative Staining EM. Any trash generated during this...
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Carbon Coating
Filing Carbon: Use the sharpener to make a graphite rod with 1cm tall and thin extending tip....
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- Electron Microscopy
- Cell Culture
- Protein Biochemistry
Morgagni Operation
!!! Radiation Safety Rules !!! Use gloves to stain your sample and insert the holder to the...
Virus Precipitation
Virus Precipitation with PEG and NaCl...
SFP Synthase (4´-phosphopantetheinyl transferase)
Phosphopantetheinyl Labeling of Proteins in vitro (P9302) Protocol Dissolve the vial of CoA substrate (50 nmol) in 50 μl of fresh DMSO to yield a labeling stock solution of 1 mM CoA substrate. Mix by vortexing for 10 minutes...
