Phosphopantetheinyl Labeling of Proteins in vitro (P9302) Protocol Dissolve the vial of CoA substrate (50 nmol) in 50 μl of fresh DMSO to yield a labeling stock solution of 1 mM CoA substrate. Mix by vortexing for 10 minutes...
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Imidazole removal - in between use/prior to storage in 20% EtOH Wash with 3-5 column volumes of 20...
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!!! Radiation Safety Rules !!! Use gloves to stain your sample and insert the holder to the...
Virus Precipitation with PEG and NaCl...
Phosphopantetheinyl Labeling of Proteins in vitro (P9302) Protocol Dissolve the vial of CoA substrate (50 nmol) in 50 μl of fresh DMSO to yield a labeling stock solution of 1 mM CoA substrate. Mix by vortexing for 10 minutes...
