How to Make a 0.5M TCEP Stock Solution

Molecular formula: C9H15O6P • HCl
Molecular weight: 286.65

  1. Dissolve 5.73 g of TCEP in 30 ml of cold molecular biology grade water in a beaker (very acidic, approximate pH of 2.5).
  2. Bring the pH of the solution to 7.0 with ~5.73 ml of 10 N NaOH or 10 N KOH.
  3. Bring the resulting solution to 40 ml with molecular biology grade water.
  4. Filter the solution using a 0.2 μm syringe filter, aliquot into 1.5 ml tubes and store at -20°C.