How to Make a 0.5M TCEP Stock Solution
Molecular formula: C9H15O6P • HCl
Molecular weight: 286.65
Step 1 - Dissolve 5.73 g of TCEP in 30 ml of cold molecular biology grade water in a beaker (very acidic, approximate pH of 2.5).
- Step 3 - Bring the pH of the solution to 7.0 with ~6.5 ml of 10 N NaOH or 10 N KOH (or ~1.13 ml per gram of TCEP).
Step 3 - Bring the resulting solution to 40 ml with molecular biology grade water.
Step 4 - Filter the solution using a 0.2 μm syringe filter, aliquot into 1.5 ml tubes and store at -20°C.More information: https://www.hamptonresearch.com/product_detail.aspx?cid=4&sid=47&pid=47