How to wash glassware for cell/tissue culture
- Disinfect your glassware, media and cells.
- Rinse the glassware with water, rub all the cell debris with brush and rinse with water x3
- Fill the glassware with water, add bleach to 10% and incubate 1 hour (30 mins to 2 hours)
- Rinse it with water x3
- Fill the glassware with water, add 7X MP Biomedicals detergent 1% and incubate at least 3 hours (better to be o/n)
- Rinse with water x3
- Send it to facility washing
- Autoclave with DI water
- Autoclave dry with thick aluminum cover.
How to disinfect cell/tissue culture surfaces
- Use 70% isopropanol to wipe all the surfaces
- Additionally use 70% ethanol if you worked with viruses
- Use 10% bleach if you spilled virus.
- First clean the surface with tissue papers
- Clean with 70% ethanol and dry with tissue papers
- Clean with 10% bleach (You should incubate metal surfaces with 10% bleach no more than 5 minutes)
- Wipe with tissue papers
- Clean with excess 70% ethanol to remove all the residual bleach
- Both ethanol and isopropanol are very effective at 70% concentration, higher and lower concentrations are less effective.
- 70% ethanol is effective against most viruses except non-enveloped viruses
- 70% isopropanol is not effective against viruses but better disinfectant than 70% ethanol for bacteria, yeast and etc.
http://chemistry.unt.edu/~verbeck/Verbeck%20Lab%20Protocol%20for%20Disinfection%20of%20Cell%20Culture%20052212%20GV.pdf
