NaOH

How to make a 10N & 100 ml NaOH Stock Solution

Molar mass: 39.997 g/mol
Formula: NaOH
Density: 2.13 g/cm³

  • Step 1 – Place a 100 ml glass bottle on a scale and add 81.23 g of distilled and filtered water into it.
  • Step 2 – Add 40 g of NaOH in 81.23 ml water. (This will bring the volume to 100 ml)

HEPES

How to make 1 M & 1 L Hepes pH:7.6 Stock Solution (Dilution of this buffer to 25mM brings the pH to 7.4)

Molar mass: 238.3012 g/mol
Formula: C8H18N2O4S
Density: 1.33 g/cm3 (predicted)

  • Add 238.3 g of Hepes and 18.965 gram of NaOH to a beaker and add distilled water to make 1 liter solution.

NaCl

How to make 5 M & 1 L NaCl Stock Solution

Molar mass: 58.44 g/mol
Formula: NaCl
Density: 2.165 g/cm3

  • Add 292.2 g of NaCl to a beaker and add 865 ml distilled water to make 1 liter solution.

Imidazole

How to make 5 M & 250 mL Imidazole Stock Solution

Molar mass: 68.077 g/mol
Formula: C3H4N2
Density: 1.23 g/cm3

  • Add 85.1 g of Imidazole to a beaker, add 155 ml distilled water, and add 25 ml concentrated HCl (32-36%) to make 250 ml solution at pH:7.5.