Glassware Washing Protocol for Cell Culture

Disinfect your glassware, media, and cells.
Rinse the glassware with water, rub all the cell debris with a brush, and rinse with water x3
Fill the glassware with water, add bleach to 10%, and incubate for 1 hour (30 mins to 2 hours)
Rinse it with water x3
Fill the glassware with water, add 7X MP Biomedicals detergent 1%, and incubate for at least 3 hours (better to be o/n)
Rinse with water x3
Send it to the washing facility
Autoclave with DI water
Autoclave dry with double layer thick aluminum cover.

Use 70% isopropanol to wipe all the surfaces
Additionally use 70% ethanol if you worked with viruses
Use 10% bleach if you spilled the virus.
- First, clean the surface with tissue papers
- Clean with 70% ethanol and dry with tissue papers
- Clean with 10% bleach (You should incubate metal surfaces with 10% bleach for no more than 5 minutes)
- Wipe with tissue papers
- Clean with excess 70% ethanol to remove all the residual bleach
Both ethanol and isopropanol are very effective at 70% concentration, and higher and lower concentrations are less effective.
70% ethanol is effective against most viruses except non-enveloped viruses
70% isopropanol is not effective against viruses but better disinfectant than 70% ethanol for bacteria, yeast and etc.

Reference:
http://chemistry.unt.edu/~verbeck/Verbeck%20Lab%20Protocol%20for%20Disinfection%20of%20Cell%20Culture%20052212%20GV.pdf